An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America

Kellie Carim; Joseph Dysthe; Michael K. Young; Kevin S. McKelvey; Michael K. Schwartz

doi:10.1007/s12686-016-0531-1

Treesearch

An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America

Formally Refereed

Authors:

Kellie Carim, Joseph Dysthe, Michael K. Young, Kevin S. McKelvey, Michael K. Schwartz

Year:

2016

Type:

Scientific Journal

Station:

Rocky Mountain Research Station

DOI:

https://doi.org/10.1007/s12686-016-0531-1

Source:

Conservation Genetics Resources. 8(3): 197-199.

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Abstract

The upper Missouri River basin in the northwestern US contains disjunct Arctic grayling (Thymallus arcticus) populations of conservation concern. To assist efforts aimed at understanding Artic grayling distribution, we developed a quantitative PCR assay to detect the presence of Arctic grayling DNA in environmental samples. The assay amplified low concentrations of Arctic grayling DNA consistently, and did not amplify non-target species, including sympatric salmonid fishes.

Keywords

Thymallus arcticus, eDNA, invasive species, qPCR

Citation

Carim, K. J.; Dysthe, J. C. S.; Young, M. K.; McKelvey, K. S.; Schwartz, M. K. 2016. An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America. Conservation Genetics Resources. 8(3): 197-199.