RAPD linkage mapping in a longleaf pine × slash pine F₁ family

Abstract

Random amplified polymorphic DNAs (RAPDs) were used to construct linkage maps of the parents of a longleaf pine (Pinus palustris Mill.) slash pine (Pinus elliottii Englm.) F₁ family. A total of 247 segregating loci [233 (1:1), 14 (3:1)] and 87 polymorphic (between-parents), but non-segregating, loci were identified. The 233 loci segregating 1: 1 (testcross configuration) were used to construct parent-specific linkage maps, 132 for the long- leaf-pine parent and 101 for the slash-pine parent. The resulting linkage maps consisted of 122 marker loci in 18 groups (three or more loci) and three pairs (1367.5 cM) for longleaf pine, and 9 1 marker loci in 13 groups and six pairs for slash pine (952.9 cM). Genome size estimates based on two-point linkage data ranged from 2348 to 2392 cM for longleaf pine, and from 2292 to 2372 cM for slash pine. Linkage of 3: 1 loci to testcross loci in each of the parental maps was used to infer further linkages within maps. as well as potentially homologous counterparts between maps. Three of the longleaf-pine linkage groups appear to be potentially homologous counterparts to four different slash-pine linkage groups. The number of heterozygous loci (previously testcross in parents) per F₁ individual, ranged from 96 to 130. With the 87 polymorphic, but non-segregating, loci that should also be heterozygous in the F₁ progeny, a maximum of 183-217 heterozygous loci could be available for mapping early height growth (EHG) loci and for applying genomic selection in backcross populations.

Citation

Kubisiak, Thomas L.; Nelson, C. Dana.; Nance, W.L.; Stine, M. 1995. RAPD linkage mapping in a longleaf pine × slash pine F₁ family. Theor. Appl. Genet. (1995) 90:1119-1127