Analysis of hindgut bacterial phyla frequency and diversity in subterranean termites exposed to chitosan-treated wood

Olanrewaju Raji; Juliet D. Tang; Telmah Telmadarrehei; Dragica Jeremic

Treesearch

Analysis of hindgut bacterial phyla frequency and diversity in subterranean termites exposed to chitosan-treated wood

Informally Refereed

Authors:

Olanrewaju Raji, Juliet D. Tang, Telmah Telmadarrehei, Dragica Jeremic

Year:

2017

Type:

Paper

Station:

Forest Products Laboratory

Source:

The International Research Group on Wood Protection, Section 1, Biology: Paper prepared for the IRG48 Scientific Conference on Wood Protection. Ghent, Belgium: 2-8.

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Abstract

The termite hindgut contains a microbial community that symbiotically aids in digestion of lignocellulosic materials. For better understanding of the dynamics of the bacteria-termite relationship, a species survey of bacterial hindgut microbes in subterranean termites (Reticulitermes flavipes: Kollar) collected from Louisville, Mississippi was performed after exposure to chitosan-treated and control (water-treated) wood samples. Total genomic DNA was isolated from termite hindguts, amplified and 16S ribosomal RNA (rRNA) gene fragments were analyzed using next-generation sequencing techniques. Twenty-six bacteria phyla were identified in both treatment groups, with five bacteria phyla showing significantly differences in abundance between the chitosan-treated and control groups. These results suggest that there was a treatment driven effect on the hindgut bacteria diversity.

Keywords

Metagenomics, Reticulitermes flavipes (Isoptera Rhinotermitidae), hindgut, bacteria, chitosan, wood

Citation

Raji, Olanrewaju; Tang, Juliet D.; Telmadarrehei, Telmah; Jeremic, Dragica. 2017. Analysis of hindgut bacterial phyla frequency and diversity in subterranean termites exposed to chitosan-treated wood. In: The International Research Group on Wood Protection, Section 1, Biology: Paper prepared for the IRG48 Scientific Conference on Wood Protection. Ghent, Belgium: 2-8.