Don't make a mista(g)ke: Is tag switching an overlooked source of error in amplicon pyrosequencing studies?

Tor Carlsen; Anders Bjornsgaard Aas; Daniel Lindner; Trude Vralstad; Trond Schumacher; Havard Kauserud

Treesearch

Don't make a mista(g)ke: Is tag switching an overlooked source of error in amplicon pyrosequencing studies?

Formally Refereed

Authors:

Tor Carlsen, Anders Bjornsgaard Aas, Daniel Lindner, Trude Vralstad, Trond Schumacher, Havard Kauserud

Year:

2012

Type:

Scientific Journal

Station:

Northern Research Station

Source:

Fungal Ecology. 5(2012): 747-749.

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Abstract

High throughput sequencing has become a powerful tool for fungal ecologists to explore the diversity and composition of fungal communities. However, various biases and errors are associated with the new sequencing techniques that must be handled properly. We here provide evidence for a source of error that has not yet been taken into account.

Citation

Carlsen, Tor; Aas, Anders Bjornsgaard; Lindner, Daniel; Vralstad, Trude; Schumacher, Trond; Kauserud, Havard. 2012. Don't make a mista(g)ke: Is tag switching an overlooked source of error in amplicon pyrosequencing studies? Fungal Ecology. 5(2012): 747-749.