Gene expression analysis of wood decay fungus Fibroporia Radiculosa grown In ACQ-treated wood

Abstract

Copper-tolerant brown-rot fungi are able todegrade wood treated with copper or copper-based wood preservatives. This research used quantitative reverse transcriptase polymerase chain reaction to explore what genes of the brown-rot fungus, Fibroporia radiculosa, were expressed when the fungus was overcoming the wood preservatives and decaying the wood. Aryl alcohol oxidase, catalase, oxalate decarboxylase 2, and copper resistance P-type ATPase pump had higher expression on alkaline copper quat type D treated wood compared with week I. In addition, two genes had high expression at week 5; glyceride hydrolase 5 and glyceride hydrolase 10 when wood strength loss was around SO%. Glyoxylate de-hydrogenase had high expression until week 8. This gene might be involved in the production of oxalate. Laccase, oxalate decarboxylase I and isocitrate yase were not differentially expressed, suggesting that these genes were not involved in the decay process of alkaline copper quat-treated wood. These results are important to understand the genes that are involved in themechanism of copper tolerance and wood decay in F.ridiculosa.

Citation

Akgul, Ayfer; Akgul, Ali; Tang, Juliet D. Diehl, Susan V. 2018. Gene expression analysis of wood decay fungus Fibroporia Radiculosa grown In ACQ-treated wood. Wood and Fiber Science. 50(2): 1-12.