Multi-omic analyses of extensively decayed Pinus contorta reveal expression of a diverse array of lignocellulose-degrading enzymes

Chiaki Hori; Jill Gaskell; Daniel Cullen; Grzegorz Sabat; Philip E. Stewart; Kathleen Lail; Yi Peng; Kerrie Barry; Igor V Grigoriev; Annegret Kohler; Laure Fauchery; Francis Martin; Carolyn A. Zeiner; Jennifer M. Bhatnagar

doi:10.1128/AEM.01133-18

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Multi-omic analyses of extensively decayed Pinus contorta reveal expression of a diverse array of lignocellulose-degrading enzymes

Formally Refereed

Authors:

Chiaki Hori, Jill Gaskell, Daniel Cullen, Grzegorz Sabat, Philip E. Stewart, Kathleen Lail, Yi Peng, Kerrie Barry, Igor V Grigoriev, Annegret Kohler, Laure Fauchery, Francis Martin, Carolyn A. Zeiner, Jennifer M. Bhatnagar

Year:

2018

Type:

Scientific Journal

Station:

Forest Products Laboratory

DOI:

https://doi.org/10.1128/AEM.01133-18

Source:

Applied and Environmental Microbiology. 84(20): 1-41.

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Abstract

Fungi play a key role cycling nutrients in forest ecosystems but the mechanisms remain uncertain. To clarify the enzymatic processes involved in wood decomposition, metatranscriptomics and metaproteomics of extensively decayed lodgepole pine by RNAseq and LC-MS/MS, respectively. Following de novo metatranscriptome assembly, 52,011 were searched for functional domains and homology to database entries.

Keywords

Pinus contorta, wood decay, white rot, brown rot

Citation

Hori, Chiaki; Gaskell, Jill; Cullen, Dan; Sabat, Grzegorz; Stewart, Philip E.; Lail, Kathleen; Peng, Yi; Barry, Kerrie; Grigoriev, Igor V.; Kohler, Annegret; Fauchery, Laure; Martin, Francis; Zeiner, Carolyn A.; Bhatnagar, Jennifer M. 2018. Multi-omic analyses of extensively decayed Pinus contorta reveal expression of a diverse array of lignocellulose-degrading enzymes. Applied and Environmental Microbiology. 84(20): 1-41.