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Abstract
We describe here recent methods for quantitative assessment of specific P. chrysosporium mRNAs in organopollutant contaminated soils and in Aspen wood chips. Magnetic capture techniques were used to rapidly purify poly(A)-RNA, and quantitative RT-PCR protocols were developed for all known lignin peroxidase (lip) and cellobiohydrolase (cbh1) genes. The methodology is extremely sensitive and highly specific. Results indicated that gene transcript patterns varied dramatically in response to substrate. In general, transcript patterns in defined media were unreliable predictors of expression in soil or wood. No apparent relationship was observed between genomic organization and transcript patterns. Results provide a framework for future investigations and help guide strain improvement programs.
Keywords
Phanerochaete chrysosporium,
Populus,
Soil fungi,
Genes,
Transcription,
Soil pollution,
Contamination,
Wood chips,
Genetic improvement,
Lignocellulose,
Strains,
Organopollutants
Citation
Vanden Wymelenberg, Amber.; Janse, Bernard.; Gaskell, Jill.; Dietrich, Diane.; Vallim, Marcelo.; Cullen, Dan. 1998. Transcript patterns of Phanerochaete chrysosporium genes in organopollutant contaminated soils and in wood. [Proceedings of the fourth meeting on the Genetics and Cellular Biology of Basidiomycetes : 1998 March 27-30, Nijmegen, The Netherlands. Horst, The Netherlands : Mushroom Experimental Station, 1998].:p. 89-93 : ill.
