Rapid 2,2'-bicinchoninic-based xylanase assay compatible with high throughput screening

Abstract

High-throughput screening requires simple assays that give reliable quantitative results. A microplate assay was developed for reducing sugar analysis that uses a 2,2'-bicinchoninic-based protein reagent. Endo-1,4-â-D-xylanase activity against oat spelt xylan was detected at activities of 0.002 to 0.011 IU ml−1. The assay is linear for sugar concentrations from 0 to 86 µg ml−1 and can also be used to assay protein concentrations (0 to 143 µg ml−1) on the same plate. A variety of temperatures and pH conditions can be used and, after incubation, the assay requires only one detection reagent and one heating step.

Citation

Kenealy, William R.; Jeffries, Thomas W. 2003. Rapid 2,2''-bicinchoninic-based xylanase assay compatible with high throughput screening. Biotechnology letters. Vol. 25 (2003): Pages 1619-1623