Treesearch

Background: Plant lignocellulosic biomass is an abundant, renewable feedstock for the production of biobased fuels and chemicals. Previously, we showed that iron can act as a co-catalyst to improve the deconstruction of lignocellulosic biomass. However, directly adding iron catalysts into biomass prior to pretreatment is diffusion limited, and increases the cost of biorefinery operations. Recently, we developed a new strategy for expressing iron-storage protein ferritin intracellularly to accumulate iron as a catalyst for the downstream deconstruction of lignocellulosic biomass. In this...

Aldehyde-functionalized cellulose nanofibers (CNFs) were applied to synthesize Pt nanoparticles (NPs) on CNF surfaces via on-site Pt ion reduction and achieve high concentration and uniform Pt NP loading. ALD could then selectively deposit TiO2 on CNFs and keep the Pt NPs uncovered due to their drastically different hydro-affinity properties. The high-temperature ALD process also simultaneously improved the crystallinity of Pt NPs and decomposed the CNF template leaving a pure anatase phase TiO2 nanofiber network decorated with high-density Pt NPs (up to 11.05 wt%). The as-prepared fibrous...

Fungal decomposition of plant cell walls (PCW) is a complex process that has diverse industrial applications and huge impacts on the carbon cycle. White rot (WR) is a powerful mode of PCW decay in which lignin and carbohydrates are both degraded. Mechanistic studies of decay coupled with comparative genomic analyses have provided clues to the enzymatic components of WR systems and their evolutionary origins, but the complete suite of genes necessary for WR remains undetermined. Here, we use phylogenomic comparative methods, which we validate through simulations, to identify shifts in gene...

This study evaluated high sulfur dioxide (SO2) loading in applying Sulfite Pretreatment to Overcome the Recalcitrance of Lignocelluloses (SPORL) to Douglas-fir forest residue (FS-10) for ethanol production through yeast fermentation. Three pretreatments were conducted at 140°C with a targeted total SO2 loading of 32 wt% on wood, or 80 g/L in the pretreatment liquor. Magnesium was used as the metal base with a targeted combined SO2 loading of 4.4 wt% on wood. A pretreatment duration of approximately 60 min was found sufficient to effectively remove the strong recalcitrance of FS-10...

Commercialization of cellulose nanomaterials (CNs) is rapidly advancing, to the benefit of many end-use product sectors, and providing information about the safe manufacturing and handling for CNs is a priority. Safety Data Sheets (SDS) are required for industrially produced materials to communicate information on their potential health, fire, reactivity, and environmental hazards, and to provide recommendations on how to safely work with these materials. Cellulose and cellulose pulp, which have widespread commercial end uses, can create nuisance dusts when dried and are required to have...

Soft-story wood-frame buildings have been recognized as a disaster preparedness problem for decades. The majority of these buildings were constructed from the 1920s to the 1960s and are prone to collapse during moderate to large earthquakes due to a characteristic deficiency in strength and stiffness in their first story. In order to propose and validate retrofit methods for these at-risk buildings, a full-scale four-story soft-story wood-frame building was constructed, retrofitted, and subjected to ground motions of various intensities. The tests were conducted to validate retrofit...

Soft-story wood-frame buildings have been recognized as a disaster preparedness problem for decades. There are tens of thousands of these multifamily three- and four-story structures throughout California and other parts of the United States. The majority were constructed between 1920 and 1970 and are prevalent in regions such as the San Francisco Bay Area in California. The NEES-Soft project was a five-university multiindustry effort that culminated in a series of full-scale soft-story wood-frame building tests to validate retrofit philosophies proposed by (1) Federal Emergency Management...

The vapor permeability (or equivalently the vapor diffusion resistance factor) and the capillary absorption coefficient are frequently used as inputs to hygrothermal or heat, air, and moisture (HAM) models. However, it has been well documented that the methods used to determine these properties are sensitive to the operator, and wide variations in the properties have been reported in round-robin testing. This paper presented an investigation into how these errors can be minimized for porous materials by different edge sealing and also looked at whether automating these techniques can...

The USDA Forest Service is required to appraise timber prior to it being offered for sale. Currently the Forest Service uses a transaction evidence based approach, but concerns have been raised about availability—both in number and applicability—of timber sales used as the basis of this approach. In addition to the problem of few sales, in certain situations a notable fraction of the sales may be made at the minimum rate. This transaction evidence system and the application of a rollback factor in setting a minimum bid can result in appraised prices declining over time in areas with low...

Wood-degrading brown rot fungi are essential recyclers of plant biomass in forest ecosystems. Their efficient cellulolytic systems, which have potential biotechnological applications, apparently depend on a combination of two mechanisms: lignocellulose oxidation (LOX) by reactive oxygen species (ROS) and polysaccharide hydrolysis by a limited set of glycoside hydrolases (GHs). Given that ROS are strongly oxidizing and nonselective, these two steps are likely segregated. A common hypothesis has been that brown rot fungi use a concentration gradient of chelated metal ions to confine ROS...