Increasing ecological inference from high throughput sequencing of fungi in the environment through a tagging approach

Abstract

High throughput sequencing methods are widely used in analyses of microbial diversity but are generally applied to small numbers of samples, which precludes charaterization of patterns of microbial diversity across space and time. We have designed a primer-tagging approach that allows pooling and subsequent sorting of numerous samples, which is directed to amplification of a region spanning the nuclear ribosomal internal transcribed spacers and partial large subunit from fungi in environmental samples.

Citation

Taylor, D.L.; Booth, M.G.; McFarland, J.W.; Herriott, I.C.; Lennon, N.J.; Nusbaum, C.; Marr, T.G. 2008. Increasing ecological inference from high throughput sequencing of fungi in the environment through a tagging approach. Molecular Ecology Resources. 8: 742-752.