Genetic mapping of Pinus flexilis major gene (Cr4) for resistance to white pine blister rust using transcriptome-based SNP genotyping

Jun-Jun Liu; Anna W. Schoettle; Richard A. Sniezko; Rona N. Sturrock; Arezoo Zamany; Holly Williams; Amanda Ha; Danelle Chan; Bob Danchok; Douglas P. Savin; Angelia Kegley

doi:10.1186/s12864-016-3079-2

Treesearch

Genetic mapping of Pinus flexilis major gene (Cr4) for resistance to white pine blister rust using transcriptome-based SNP genotyping

Formally Refereed

Authors:

Jun-Jun Liu, Anna W. Schoettle, Richard A. Sniezko, Rona N. Sturrock, Arezoo Zamany, Holly Williams, Amanda Ha, Danelle Chan, Bob Danchok, Douglas P. Savin, Angelia Kegley

Year:

2016

Type:

Scientific Journal

Station:

Rocky Mountain Research Station

DOI:

https://doi.org/10.1186/s12864-016-3079-2

Source:

BMC Genomics. 17: 753.

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Abstract

Linkage of DNA markers with phenotypic traits provides essential information to dissect clustered genes with potential phenotypic contributions in a target genome region. Pinus flexilis E. James (limber pine) is a keystone five-needle pine species in mountain-top ecosystems of North America. White pine blister rust (WPBR), caused by a non-native fungal pathogen Cronartium ribicola (J.C. Fisch.), has resulted in mortality in this conifer species and is still spreading through the distribution. The objective of this research was to develop P. flexilis transcriptome-wide single nucleotide polymorphism (SNP) markers using RNA-seq analysis for genetic mapping of the major gene (Cr4) that confers complete resistance to C. ribicola.

Keywords

disease resistance, genetic map, limber pine, transcriptome de novo assembly, white pine blister rust

Citation

Liu, Jun-Jun; Schoettle, Anna W.; Sniezko, Richard A.; Sturrock, Rona N.; Zamany, Arezoo; Williams, Holly; Ha, Amanda; Chan, Danelle; Danchok, Bob; Savin, Douglas P.; Kegley, Angelia. 2016. Genetic mapping of Pinus flexilis major gene (Cr4) for resistance to white pine blister rust using transcriptome-based SNP genotyping. BMC Genomics. 17: 753.