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- The Acetivibrio clariflavus (basonym: Clostridium clariflavum) glycoside hydrolase family 30 cellulosomal protein encoded by the Clocl_1795 gene was highly represented during growth on cellulosic substrates. In this report, the recombinantly expressed protein has been characterized and shown to be a non-reducing terminal (NRT)-specific xylobiohydrolase (AcXbh30A). Biochemical function, optimal biophysical parameters, and phylogeny were investigated. The findings indicate that AcXbh30A strictly cleaves xylobiose from the NRT up until an α-1,2-linked glucuronic acid (GA)-decorated xylose if...AuthorsCasey Crooks, Nathan J. Bechle, Franz St. JohnKeywordsSourceFrontiers in Molecular BiosciencesYear2021
- The 2nd Mass Timber Research Needs Assessment Workshop was held on November 13-14, 2018 at the US Forest Service, Forest Products Laboratory (FPL). The purpose of the workshop was to convene a group of experts on cross laminated timber and mass timber to develop a list of prioritized research needs for the North American mass timber industry. The workshop had over 100 attendees including design professionals, academics, industry leaders, and government employees. The attendees generated a list of over 117 research needs. After the workshop, the list of 117 research needs was prioritized...AuthorsSamuel L. Zelinka, Tom Williamson, Karen Martinson, Michael A. RitterKeywordsSourcepaper in proceedingsYear2021
- Water vapor sorption is an important characteristic of wood as an engineering material. In addition to affecting engineering properties such as the heat capacity, thermal conductivity, strength, stiffness, and dimensions, moisture is fundamental to many wood degradation mechanisms including fungal decay. Water vapor sorption isotherms have been gaining increasing attention in the wood literature as more laboratories adopt automated sorption balances, frequently called dynamic vapor sorption (DVS) analyzers. With DVS, the collection of sorption isotherms can be automated, completely...AuthorsSamuel L. Zelinka, Emil E. Thybring, Samuel V. GlassKeywordsSourcepaper in proceedingsYear2021
- The fire resistance of a structural building member includes its ability to survive a specified fire without loss of its loadbearing function. For glue laminated timber columns, fire resistance is determined by either subjecting a structural member to a standard fire test or by using one of two accepted calculation methods. For wood structural members, the calculation methods rely on char rates obtained from numerous standard fire tests. The existing calculation methods are limited under United States building codes to calculating fire resistance ratings of 120 minutes or less. However,...AuthorsLaura Hasburgh, Keith Bourne, David BarberKeywordsSourceWorld Conference on Timber Engineering proceedingsYear2021
- This paper examines how the moisture conditioning method affects the electrical conductance of wood. A widely used dataset was acquired by Stamm in 1929 who used a method of conditioning where water-saturated wood specimens were partially dried, sealed and left for a period of time for moisture to be redistributed before the electrical conductance was measured. However, more recent measurements combined conditioning above saturated salt solutions and pressure plate/pressure membrane techniques to obtain equilibrium moisture contents at constant relative humidity levels in the full moisture...AuthorsMaria Fredriksson, Emil Engelund Thybring, Samuel L. ZelinkaSourceHolzforschungYear2021
- Deep eutectic solvent treatment to overcome lignocellulose resistance to deconstruction has great potential for the production of biofuels and bioproducts. Most investigations have been focused on optimizing the sugar yield from specific feedstocks. However, little is known about the interaction between deep eutectic solvent and cellulose during pretreatment, post-treatment cellulose accessibility to enzymes, and lignin fractionation. We evaluated four deep eutectic solvents that consisted of choline chloride and biomass-derived polyols (ethylene glycol, glycerol, xylitol, and sorbitol)....AuthorsMd Anwar Hossain, Mohammad Shahinur Rahaman, Daniel J. Yelle, Hong Shang, Zhihui Sun, Scott Renneckar, Jie Dong, Sarttrawut Tulaphol, Noppadon SathitsuksanohSourceIndustrial Crops and ProductsYear2021
- Atmospheric forcing and interactions between the fire and atmosphere are primary drivers of wildland fire behavior. The atmosphere is known to be a chaotic system that, although deterministic, is very sensitive to small perturbations to initial conditions. We assume that as a result of the tight coupling between fire and atmosphere; wildland fire behavior, in turn, should also be sensitive to perturbations in atmospheric initial conditions. Observations suggest that low intensity prescribed fire, in particular, is susceptible to small perturbations in the wind field, which can significantly...AuthorsAlexandra K. Jonko, Kara Yedinak, Juliana L. Conley, Rodman R. LinnKeywordsSourceJournal of Geophysical Research: AtmospheresYear2021
- Biocatalysis using molecular oxygen as the electron acceptor has significant potential for selective oxidations at low cost. However, oxygen is poorly soluble in water, and its slow rate of mass transfer in the aqueous phase is a major obstacle, even for laboratory-scale syntheses. Oxygen transfer can be accelerated by vigorous mechanical methods, but these are often incompatible with biological catalysts. Gentler conditions can be achieved with shallow, high surface area bag reactors that are designed for single use and generally for specialized cell culture applications. As a less-expensi...AuthorsMichael Mozuch, Kolby Hirth, Thomas J. Schwartz, Philip J. KerstenKeywordsSourceApplied Biochemistry and BiotechnologyYear2021
- This paper is a review of published studies involving the use of ground penetrating radar (GPR) on wood structures. It also contains background information to help the reader understand how GPR functions. The use of GPR on wood structures began to grow in popularity at the turn of the millennium. GPR has many characteristics that make it attractive as an inspection tool for wood: it is faster than many acoustic and stress wave techniques; it does not require the use of a couplant; while it can also detect the presence of moisture. Moisture detection is of prime concern, and several...AuthorsBrunela Pollastrelli Rodrigues, Christopher Adam Senalik, Xi Wu, James WackerKeywordsSourceForestsYear2021
- Southern yellow pine (SYP) is one of the most used softwood species in the world. Most of this raw material come from fast-grown plantation trees. It is of interest to determine if SYP clear wood properties may have changed over the long term, in particular whether such properties may have declined. Herein, specific gravity (SG), ultimate compression strength parallel to grain (UCS||), and UCS perpendicular to grain (UCS⊥) from three samples were compared: Sample 1 tested in 2014; Sample 2 from molding and millwork producers tested in 2017–2019; and Sample 3 from a study conducted in the...AuthorsRubin Shmulsky, Frederico José Nistal França, J. Tedrick Ratcliff, Benjamin Farber, Christopher Adam Senalik, Robert J. Ross, R. Daniel. SealeKeywordsSourceForest Products JournalYear2021